LOCUS       40924_23669       12029 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB704 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12029)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12029)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12029)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    328..452
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        489..519
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             573..1229
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1574..1876
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    1920..2044
                     /label=attR2
                     /note="attR2"
     protein_bind    complement(1920..2044)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2049..2765
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      2793..3045
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3079..3095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3103..3119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3127..3157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3172..3193)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3229..3481)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3519..4814)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61168.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3519..4814)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4866..5045)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5551..5575)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6096..6884
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7133..7721
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7907..8047)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8391..8585)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8654..9724)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10156..10782)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggc tctaatcaca agtttgtaca aaaaagctga acgagaaacg
      361 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      421 tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc gcattaggca
      481 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      541 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      601 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      661 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
      721 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
      781 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
      841 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
      901 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
      961 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     1021 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     1081 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     1141 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     1201 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca
     1261 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     1321 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     1381 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     1441 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     1501 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     1561 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     1621 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     1681 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     1741 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     1801 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     1861 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     1921 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     1981 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     2041 tggtgatcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2101 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2161 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2221 ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg cttcagccgc taccccgacc
     2281 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2341 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2401 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2461 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2521 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2581 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2641 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2701 acatggtcct gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt
     2761 acaagtaaag cggcccgagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     2821 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     2881 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2941 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     3001 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttggttccgg
     3061 aaccaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3181 ctaactcaca ttaggctgaa ttaggcgcgc ctatttctat taattcccga tctagtaaca
     3241 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
     3301 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
     3361 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
     3421 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
     3481 cggggaaatt cgcctcgagt gacttctctg attgcagact atttatacca accagcaaga
     3541 aagtgtcgaa gtatgaagca gaaggcgcag gctagagcct ggaaaacgag aagatggatg
     3601 caaagagact tttctgattt aggaccaaac aacctcaagt aaacattgac gagtgtccca
     3661 tcttttgtgc cggtcagtaa tccggtagca ggatcatacc ttgggagacg atggcgcgga
     3721 catttcacaa tcccagcaag ctgaggaagc gataatagaa ggttcagaac ttgaggaaga
     3781 aagaagatga ggagggtttc actgaagtga cccagaatac caacaactgc catagtcatt
     3841 ccggcgaaga ctgtgtaagt gtctccaaca aaaactgaag aaggatacca attgtaagca
     3901 agcattgcca aggatgtggc catcagcggt tgagtaagaa atatcgagaa ggcatgagct
     3961 tggtgatact cgggatcaac agatgctccg atttgcataa cgttgtgtat caggatagca
     4021 gcggcaataa caactgtttg accgatttca aggccattca gaccagcgtg gatgttaata
     4081 gagtttgtac aaaagacagc aagtagcccc atatataact tatatatcct tcctagatta
     4141 agcacttcca agccaatata agcaacaaga ggttttggta taacaatagt tgtatgacca
     4201 gcataagcca tcaaaagtgg aagggtagca aaagatggca ggacaagttt tactctccaa
     4261 ggcacatcaa ggacatcatc tacaaatcca agcaaaatca tgaagcaaat agatgctagt
     4321 gctgcattat actccacaag ccaattagaa tcttcagtga agttaaaata ctgaaatatt
     4381 atcgccacga tcaaaaagac aataccaaca acaataccca atgactctgg cactttaata
     4441 tctccttgag gcgtgcctct tttgttgata tcaaaaccaa acatgttacg tctcagaacg
     4501 tatctggcag taacaggaat cattttgaga gtgacgaaaa acccaaccaa gctaagcccc
     4561 gcgttaatta gaatcgatcg cttgagttca ttatcaacct tgtagtgaaa acagagaagg
     4621 tatagataga gcgaacagag aagagtagag atcacgaaga tcactcctaa tttcggcggc
     4681 gcgagacgaa attcctcacc ggaagcagaa accgtctttc gggtcatctt ctgctccgat
     4741 ggcgctgagt tgggttccga cgggtccggt ttgttgggaa ttgagatcga tgaagctctc
     4801 ttacgagctg ccatatctta ttgccccccg ggatcagatc tggattgaga gtgaatatga
     4861 gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa
     4921 atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac
     4981 gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc
     5041 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     5101 tgactccctt aattctccgc tcatgatctt gatcccctgc gccatcagat ccttggcggc
     5161 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     5221 ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc
     5281 ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag
     5341 tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc
     5401 ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagctga tccgtcgaga
     5461 ttaatagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa
     5521 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     5581 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     5641 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     5701 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     5761 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     5821 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     5881 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     5941 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     6001 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     6061 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
     6121 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     6181 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     6241 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     6301 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     6361 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     6421 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     6481 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     6541 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     6601 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     6661 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     6721 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     6781 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     6841 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
     6901 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
     6961 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
     7021 cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg
     7081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7261 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7321 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7381 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7441 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7501 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7561 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7621 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7681 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7741 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7801 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7861 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     7921 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     7981 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     8041 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8101 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8161 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     8221 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     8281 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     8341 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     8401 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     8461 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     8521 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     8581 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     8641 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     8701 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     8761 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     8821 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     8881 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     8941 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     9001 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     9061 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     9121 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     9181 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
     9241 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
     9301 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
     9361 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
     9421 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
     9481 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
     9541 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
     9601 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
     9661 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
     9721 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
     9781 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     9841 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     9901 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
     9961 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    10021 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    10081 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    10141 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    10201 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    10261 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    10321 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    10381 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    10441 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    10501 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    10561 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    10621 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    10681 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    10741 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    10801 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    10861 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    10921 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    10981 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    11041 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    11101 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    11161 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    11221 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    11281 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    11341 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    11401 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    11461 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    11521 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    11581 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    11641 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    11701 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    11761 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    11821 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    11881 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    11941 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    12001 cacatacaaa tggacgaacg gataaacct
//
